PAPOLA

PAPOLA
Identificadores
Nomes alternativos	PAPOLA
IDs externos	OMIM: 605553 HomoloGene: 23389 GeneCards: PAPOLA
Ontologia genética
Função molecular	• transferase activity; • nucleotide binding; • nucleotidyltransferase activity; • manganese ion binding; • metal ion binding; • GO:0001948, GO:0016582 ligação a proteínas plasmáticas; • RNA binding; • ATP binding; • magnesium ion binding; • polynucleotide adenylyltransferase activity;
Componente celular	• citoplasma; • cariolinfa; • núcleo celular;
Processo biológico	• mRNA splicing, via spliceosome; • termination of RNA polymerase II transcription; • RNA 3'-end processing; • mRNA processing; • mRNA polyadenylation; • mRNA 3'-end processing; • regulation of mRNA 3'-end processing; • RNA polyadenylation;
	Sources:Amigo / QuickGO
Padrão de expressão RNA
	; ; ; ;
	Mais dados de referência de expressão
Ortólogos
	10914
	n/a
	ENSG00000090060
	n/a
	P51003
	n/a
NM_001252006; NM_001252007; NM_001293627; NM_001293628; NM_001293632;
	NM_032632; NM_001363662; NM_001363664; NM_001363665; NM_001363666
	n/a
NP_001238935; NP_001238936; NP_001280556; NP_001280557; NP_001280561;
	NP_116021; NP_001350591; NP_001350593; NP_001350594; NP_001350595
	n/a
	Wikidata
Ver/Editar Humano

A poli(A) polimerase alfa é uma enzima que em humanos é codificada pelo gene PAPOLA.^[2]^[3]^[4]

PAPOLA se liga ao FIP1L1 (fator que interage com PAPOLA e CPSF1), uma subunidade do complexo de clivagem e fator de especificidade da poliadenilação subunidade 1 (CPSF1). Este complexo poliadenila a extremidade 3 ' dos mRNAs precursores (pré-mRNA) (ver CPSF). O CPSF1 é uma proteína de processamento de RNA que se liga a seqüências ricas em uracil no pré-mRNA, se liga e estimula a atividade do polinucleotídeo adenililtransferase da POPOLA e, portanto, adiciona resíduos de adenililo ao pré-mRNA. Essa ação poli-adenililo aumenta a maturação e o movimento do pré-mRNA do núcleo para o citoplasma, enquanto aumenta drasticamente a estabilidade do mRNA formado a partir do pré-mRNA: FIP1L1 é um fator de processamento final do pré-mRNA 3'. O gene de fusão FIP1L1 entre ele e os genes do receptor do fator de crescimento derivado de plaquetas, alfa (PGDFRA) ou do receptor alfa do ácido retinoico (RARA) são causas de certas doenças humanas associadas a níveis patologicamente aumentados de eosinófilos no sangue e/ou leucemias.^[5]

Referências

↑ «Human PubMed Reference:»
↑ Thuresson AC, Astrom J, Astrom A, Gronvik KO, Virtanen A. «Multiple forms of poly(A) polymerases in human cells». Proc Natl Acad Sci U S A. 91: 979–83. PMC 521437. PMID 8302877. doi:10.1073/pnas.91.3.979
↑ Yamauchi T, Sugimoto J, Hatakeyama T, Asakawa S, Shimizu N, Isobe M. «Assignment of the human poly(A) polymerase (PAP) gene to chromosome 14q32.1-q32.2 and isolation of a polymorphic CA repeat sequence». J Hum Genet. 44: 253–5. PMID 10429366. doi:10.1007/s100380050154
↑ «Entrez Gene: PAPOLA poly(A) polymerase alpha»
↑ «FIP1L1 factor interacting with PAPOLA and CPSF1 [Homo sapiens (human)] - Gene - NCBI»

Bibliografia[editar | editar código-fonte]

Weichs an der Glon C, Ashe M, Eggermont J, Proudfoot NJ (1993). «Tat-dependent occlusion of the HIV poly(A) site.». EMBO J. 12 (5): 2119–28. PMC 413433. PMID 8491200. doi:10.1002/j.1460-2075.1993.tb05860.x
Pendurthi UR, Alok D, Rao LV (1997). «Binding of factor VIIa to tissue factor induces alterations in gene expression in human fibroblast cells: up-regulation of poly(A) polymerase.». Proc. Natl. Acad. Sci. U.S.A. 94 (23): 12598–603. PMC 25051. PMID 9356495. doi:10.1073/pnas.94.23.12598
Varani L, Gunderson SI, Mattaj IW, et al. (2000). «The NMR structure of the 38 kDa U1A protein - PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein.». Nat. Struct. Biol. 7 (4): 329–35. PMID 10742179. doi:10.1038/74101
Mouland AJ, Coady M, Yao XJ, Cohen EA (2002). «Hypophosphorylation of poly(A) polymerase and increased polyadenylation activity are associated with human immunodeficiency virus type 1 Vpr expression.». Virology. 292 (2): 321–30. PMID 11878934. doi:10.1006/viro.2001.1261
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). «Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.». Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. PMC 139241. PMID 12477932. doi:10.1073/pnas.242603899
Kim H, Lee JH, Lee Y (2003). «Regulation of poly(A) polymerase by 14-3-3epsilon.». EMBO J. 22 (19): 5208–19. PMC 204469. PMID 14517258. doi:10.1093/emboj/cdg486
Ota T, Suzuki Y, Nishikawa T, et al. (2004). «Complete sequencing and characterization of 21,243 full-length human cDNAs.». Nat. Genet. 36 (1): 40–5. PMID 14702039. doi:10.1038/ng1285
Bouwmeester T, Bauch A, Ruffner H, et al. (2004). «A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.». Nat. Cell Biol. 6 (2): 97–105. PMID 14743216. doi:10.1038/ncb1086
Kaufmann I, Martin G, Friedlein A, et al. (2005). «Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.». EMBO J. 23 (3): 616–26. PMC 1271804. PMID 14749727. doi:10.1038/sj.emboj.7600070
Dettwiler S, Aringhieri C, Cardinale S, et al. (2005). «Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization.» (PDF). J. Biol. Chem. 279 (34): 35788–97. PMID 15169763. doi:10.1074/jbc.M403927200
Colland F, Jacq X, Trouplin V, et al. (2004). «Functional proteomics mapping of a human signaling pathway.». Genome Res. 14 (7): 1324–32. PMC 442148. PMID 15231748. doi:10.1101/gr.2334104
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). «Large-scale characterization of HeLa cell nuclear phosphoproteins.». Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. PMC 514446. PMID 15302935. doi:10.1073/pnas.0404720101
Gerhard DS, Wagner L, Feingold EA, et al. (2004). «The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).». Genome Res. 14 (10B): 2121–7. PMC 528928. PMID 15489334. doi:10.1101/gr.2596504
Rual JF, Venkatesan K, Hao T, et al. (2005). «Towards a proteome-scale map of the human protein-protein interaction network.». Nature. 437 (7062): 1173–8. PMID 16189514. doi:10.1038/nature04209

[1] «Human PubMed Reference:»

[pmid8302877-2] Thuresson AC, Astrom J, Astrom A, Gronvik KO, Virtanen A. «Multiple forms of poly(A) polymerases in human cells». Proc Natl Acad Sci U S A. 91: 979–83. PMC 521437. PMID 8302877. doi:10.1073/pnas.91.3.979

[pmid10429366-3] Yamauchi T, Sugimoto J, Hatakeyama T, Asakawa S, Shimizu N, Isobe M. «Assignment of the human poly(A) polymerase (PAP) gene to chromosome 14q32.1-q32.2 and isolation of a polymorphic CA repeat sequence». J Hum Genet. 44: 253–5. PMID 10429366. doi:10.1007/s100380050154

[entrez-4] «Entrez Gene: PAPOLA poly(A) polymerase alpha»

[5] «FIP1L1 factor interacting with PAPOLA and CPSF1 [Homo sapiens (human)] - Gene - NCBI»

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